肥大细胞对重组口蹄疫病毒VP1-VP4蛋白的模式识别效应

doi:10.11843/j.issn.0366-6964.2015.09.021

Abstract

Abstract:

The objective of this study was designed to investigate the effectiveness of mast cell pattern recognition of recombinant VP1-VP4 of foot-and-mouth disease virus(FMDV).To this end，murine mast cell line P815 were pulsed with the recombinant VP1-VP4 of FMDV after inhibitors of Toll-like receptor 2/4(OxPAPC)，mannose receptors(Mannan)，and scavenger receptors(Sennoside B) were administered to murine mast cell line，respectively.Degranulation of P815 mast cells stained with toluidine blue was observed at the given time.And the levels of TNF-α in P815 mast cell culture supernatants were determined by ELISA at different timepoints.It was showed that the number of degranulated P815 mast cells with administration of scavenger receptor inhibitor was significantly lower than that of cells pulsed with recombinant VP1-VP4 protein at 15 min and 30 min(P<0.001).Intriguingly，the TNF-α release from the mast cell line activated by recombinant VP1-VP4 at 24 h was significantly blocked with three inhibitors(P<0.01)，of which，mannan，a mannose receptor inhibitor，showed the strongest inhibition on TNF-α release.Our data indicate that murine mast cell line P815 degranulation triggered by recombinant VP1-VP4 was predominantly mediated via the recognition of scavenger receptors,while the TNF-α release from the activated mast cells was constitutively initiated with the recognition of mannose receptors，TLR2，and TLR4.Of note，recognition of recombinant VP1-VP4 through mannose receptors plays a predominant role in triggering TNF-α release from P815 mast cells activated by recombinant VP1-VP4.

CLC Number:

S852.4

LI Li-min，WANG Yan，CUI Bei-bei，LIN Min，ZHANG Lei，ZUO Yu-zhu，WANG Jia-xin. The Effectiveness of Mast Cell Pattern Recognition of Recombinant VP1-VP4 of Foot-and-mouth Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1644-1649.

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The Effectiveness of Mast Cell Pattern Recognition of Recombinant VP1-VP4 of Foot-and-mouth Disease Virus

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